A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes-Reference-Cited by-同舟云学术

A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes

Published:2022 Issue:44 Volume:20 Page:8618-8622
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Bai Chunsen¹^ORCID,Klimkowski Piotr¹,Jin Cheng¹,Kuchlyan Jagannath¹^ORCID,El-Sagheer Afaf H.¹²,Brown Tom¹^ORCID

Affiliation:

1. Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK

2. Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt

Abstract

A novel phosphoramidite (AP-C3 dT) containing an alkyne and amine has been synthesized, enabling orthogonal labelling for combination oligonucleotides probes.

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/OB/D2OB01899C

Reference29 articles.

1. Methods for Genotyping Single Nucleotide Polymorphisms

2. Fluorescence based strategies for genetic analysis

3. [3] Illumina Universal Bead Arrays

4. Next-generation DNA sequencing

5. Molecular Beacons: Probes that Fluoresce upon Hybridization

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