Affiliation:
1. Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Flemingovo nám. 2 16000 Prague 6 Czech Republic
2. Department of Organic Chemistry Faculty of Science Charles University Hlavova 8 12843 Prague 2 Czech Republic
Abstract
AbstractA modified 2′‐deoxycytidine triphosphate derivative (dCTOTP) bearing a thiazole orange moiety tethered via an oligoethylene glycol linker was designed and synthesized. The nucleotide was incorporated into DNA by DNA polymerases in vitro as well as in live cells. Upon incorporation of dCTOTP into DNA, the thiazole orange moiety exhibited a fluorescence lifetime that differed significantly from the non‐incorporated (i.e. free and non‐covalently intercalated) forms of dCTOTP. When dCTOTP was delivered into live U‐2 OS cells using a synthetic nucleoside triphosphate transporter, it allowed us to distinguish and monitor cells that were actively synthesizing DNA in real time, from the very first moments after the treatment. We anticipate that this probe could be used to study chromatin organization and dynamics.
Funder
Grantová Agentura České Republiky
Subject
General Chemistry,Catalysis