Embedding a membrane protein into an enveloped artificial viral replica-Reference-Cited by-同舟云学术

Embedding a membrane protein into an enveloped artificial viral replica

Published:2022 Issue:2 Volume:3 Page:231-241
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Furukawa Hiroto¹,Inaba Hiroshi¹²^ORCID,Sasaki Yoshihiro³^ORCID,Akiyoshi Kazunari³,Matsuura Kazunori¹²^ORCID

Affiliation:

1. Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Koyama-Minami 4-101, Tottori 680-8552, Japan

2. Centre for Research on Green Sustainable Chemistry, Tottori University, Koyama-Minami 4-101, Tottori 680-8552, Japan

3. Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

Abstract

We demonstrate the embedding membrane protein, Cx43, on the enveloped artificial viral capsid using a cell-free expression system. The embedding of Cx43 on the envelope was evaluated by detection with anti-Cx43 antibody using FCS and TEM.

Funder

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CB/D1CB00166C

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