Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step-Reference-Cited by-同舟云学术

Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step

Published:2021 Issue:22 Volume:12 Page:7735-7745
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Auhim Husam Sabah¹²³⁴⁵,Grigorenko Bella L.⁶⁷⁸⁹¹⁰,Harris Tessa K.¹²³⁴⁵,Aksakal Ozan E.¹²³⁴⁵,Polyakov Igor V.⁶⁷⁸⁹¹⁰,Berry Colin¹²³⁴⁵^ORCID,Gomes Gabriel dos Passos¹¹¹²¹³¹⁴¹⁵^ORCID,Alabugin Igor V.¹⁶¹⁷¹⁸¹⁹^ORCID,Rizkallah Pierre J.²⁰³⁵^ORCID,Nemukhin Alexander V.⁶⁷⁸⁹¹⁰^ORCID,Jones D. Dafydd¹²³⁴⁵^ORCID

Affiliation:

1. School of Biosciences

2. Molecular Biosciences Division

3. Cardiff University

4. Cardiff

5. UK

6. Chemistry Department

7. Lomonosov Moscow State University

8. Moscow

9. Russian Federation

10. Emanuel Institute of Biochemical Physics

11. Department of Chemistry

12. University of Toronto

13. Toronto

14. Canada

15. Department of Computer Science

16. Department of Chemistry and Biochemistry

17. Florida State University

18. Tallahassee

19. USA

20. School of Medicine

Abstract

Fluorescent proteins (FPs) have revolutionised the life sciences, but the chromophore maturation mechanism is still not fully understood. Here we photochemically trap maturation at a crucial stage and structurally characterise the intermediate.

Funder

Engineering and Physical Sciences Research Council

Higher Committee for Education Development in Iraq

Russian Science Foundation

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC06693A

Reference74 articles.