Histidine-assisted reduction of arylnitrenes upon photo-activation of phenyl azide chromophores in GFP-like fluorescent proteins-Reference-Cited by-同舟云学术

Histidine-assisted reduction of arylnitrenes upon photo-activation of phenyl azide chromophores in GFP-like fluorescent proteins

Published:2024 Issue:2 Volume:22 Page:337-347
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Grigorenko Bella L.¹²^ORCID,Khrenova Maria G.¹³^ORCID,Jones D. Dafydd⁴^ORCID,Nemukhin Alexander V.¹²^ORCID

Affiliation:

1. Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation

2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russian Federation

3. Bach Institute of Biochemistry, Moscow, Russian Federation

4. School of Biosciences, Molecular Biosciences Division, Cardiff University, Cardiff, UK

Abstract

A photochemical transformation to arylnitrene occurs in GFP variants with a non-canonical amino acid residue, p-azidophenylalanine, that replaces Tyr66. The arylnitrene reduction is coupled with the oxidation of the nearby His148 side chain.

Funder

Russian Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2024/OB/D3OB01450A

Reference41 articles.

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5. Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence