Cell pairing and polyethylene glycol (PEG)-mediated cell fusion using two-step centrifugation-assisted single-cell trapping (CAScT)-Reference-Cited by-同舟云学术

Cell pairing and polyethylene glycol (PEG)-mediated cell fusion using two-step centrifugation-assisted single-cell trapping (CAScT)

Published:2018 Issue:7 Volume:18 Page:1113-1120
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Huang Lu¹²³⁴⁵,Chen Yin⁶²⁴,Huang Wei⁷⁸⁹³⁴,Wu Hongkai¹²³⁴⁵^ORCID

Affiliation:

1. Shenzhen Research Institute

2. The Hong Kong University of Science and Technology

3. Shenzhen

4. China

5. Department of Chemistry

6. Division of Biomedical Engineering

7. Department of Biology

8. Shenzhen Key Laboratory of Cell Microenvironment

9. South University of Science and Technology of China

Abstract

We present a strategy of two-step centrifugation-assisted single-cell trapping (CAScT) for heterotypic cell pairing and demonstrate its compatibility with polyethylene glycol (PEG)-mediated cell fusion.

Funder

Research Grants Council, University Grants Committee

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2018/LC/C7LC01131H

Reference45 articles.

1. Microfluidic control of cell pairing and fusion

2. Deformability-based microfluidic cell pairing and fusion

3. A microfluidic approach towards hybridoma generation for cancer immunotherapy

4. A Cell Electrofusion Chip for Somatic Cells Reprogramming

5. Optically-Induced Cell Fusion on Cell Pairing Microstructures

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