Fluorescence turn-on detection of a protein through the displaced single-stranded DNA bindingprotein binding to a molecular beacon-Reference-Cited by-同舟云学术

Fluorescence turn-on detection of a protein through the displaced single-stranded DNA bindingprotein binding to a molecular beacon

Published:2011 Issue:19 Volume:47 Page:5485-5487
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Tang Dan¹²³⁴⁵,Liao Dongli¹²³⁴⁵,Zhu Qiankun¹²³⁴⁵,Wang Fangyuan¹²³⁴⁵,Jiao Huping¹²³⁴⁵,Zhang Yujing⁶⁷⁸⁹⁵,Yu Cong¹²³⁴⁵

Affiliation:

1. State Key Laboratory of Electroanalytical Chemistry

2. Changchun Institute of Applied Chemistry

3. Chinese Academy of Sciences

4. Changchun 130022

5. P. R. China

6. Department of Animal Biotechnology

7. College of Animal Science and Veterinary Medicine

8. Jilin University

9. Changchun 130062

Abstract

A new approach for the highly sensitive and selective sensing of a protein (lysozyme) based on a nucleic acid aptamer, a molecular beacon, and the single-stranded DNA binding (SSB) protein has been developed.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2011/CC/C1CC10316D

Reference24 articles.

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2. A Microdevice with Integrated Liquid Junction for Facile Peptide and Protein Analysis by Capillary Electrophoresis/Electrospray Mass Spectrometry

3. Mass spectrometry analysis of CD4-associating proteins using affinity chromatography and affinity tag-mediated purification of tryptic peptides

4. Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection

5. SERS opens a new way in aptasensor for protein recognition with high sensitivity and selectivity

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