Inline electrochemical reduction of NISTmAb for middle-up subunit liquid chromatography-mass spectrometry analysis-Reference-Cited by-同舟云学术

Inline electrochemical reduction of NISTmAb for middle-up subunit liquid chromatography-mass spectrometry analysis

Published:2021 Issue:21 Volume:146 Page:6547-6555
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Morgan Tomos E.¹,Jakes Craig¹²,Brouwer Hendrik-Jan³^ORCID,Millán-Martín Silvia¹,Chervet Jean-Pierre³,Cook Ken⁴,Carillo Sara¹,Bones Jonathan¹²^ORCID

Affiliation:

1. Characterisation and Comparability Laboratory, NIBRT – the National Institute for bioprocessing research and training, Foster Avenue, Mount Merrion, Blackrock, Co., Dublin, A94 X099, Ireland

2. School of Chemical Engineering and Bioprocessing, University College of Dublin, Belfield, Dublin 4, Ireland

3. Antec Scientific, Industrieweg 12, 2382NV Zoeterwoude, The Netherlands

4. Thermo Fisher Scientific, Hemel Hempstead, Herts, HP2 7GE, UK

Abstract

Complete inline reduction of NISTmAb disulfide bonds using an electrochemical cell coupled to LC-MS analysis, reduction is carried out without the use of traditional reducing agents or enzymes.

Funder

Thermo Fisher Scientific

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN01184G

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