Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system-Reference-Cited by-同舟云学术

Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system

Published:2021 Issue:17 Volume:146 Page:5271-5279
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Cai Qiqi¹,Wang Rui²^ORCID,Qiao Zhaohui¹^ORCID,Yang Wenge¹

Affiliation:

1. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China

2. State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200432, China

Abstract

An ultrasensitive, rapid, and visual detection platform for Salmonella Typhimurium based on the bio-barcode assay and recombinase polymerase amplification (RPA) coupled with a CRISPR-Cas12a cleavage system is presented.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

Zhejiang Province Public Welfare Technology Application Research Project

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00717C

Reference42 articles.

1. HumanSalmonellaand Concurrent Decreased Susceptibility to Quinolones and Extended-Spectrum Cephalosporins