Metabolism in action: stable isotope probing using vibrational spectroscopy and SIMS reveals kinetic and metabolic flux of key substrates-Reference-Cited by-同舟云学术

Metabolism in action: stable isotope probing using vibrational spectroscopy and SIMS reveals kinetic and metabolic flux of key substrates

Published:2021 Issue:5 Volume:146 Page:1734-1746
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Chisanga Malama¹²³⁴⁵,Muhamadali Howbeer⁶⁷⁸⁹¹⁰,McDougall Danielle¹¹¹³⁴⁵,Xu Yun⁶⁷⁸⁹¹⁰^ORCID,Lockyer Nicholas¹¹¹³⁴⁵^ORCID,Goodacre Royston⁶⁷⁸⁹¹⁰^ORCID

Affiliation:

1. Department of Chemistry

2. Manchester Institute of Biotechnology

3. University of Manchester

4. Manchester

5. UK

6. Department of Biochemistry and Systems Biology

7. Institute of Systems

8. Molecular and Integrative Biology

9. University of Liverpool

10. Liverpool

11. Photon Science Institute

Abstract

Raman and infrared are used to measure dynamics in bacterial metabolism by employing stable isotope probing with temporal acquisition. The work is complemented by SIMS for confirmation of ¹³C incorporation into bacterial biomass.

Funder

Commonwealth Scholarship Commission

European Commission

University of Liverpool

Biotechnology and Biological Sciences Research Council

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D0AN02319A

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