Affiliation:
1. CEH Oxford, Oxford OX1 3SR, United Kingdom
Abstract
ABSTRACT
Identifying microorganisms responsible for recognized environmental processes remains a great challenge in contemporary microbial ecology. Only in the last few years have methodological innovations provided access to the relationship between the function of a microbial community and the phylogeny of the organisms accountable for it. In this study stable-isotope-labeled [
13
C]phenol was fed into a phenol-degrading community from an aerobic industrial bioreactor, and the
13
C-labeled RNA produced was used to identify the bacteria responsible for the process. Stable-isotope-labeled RNA was analyzed by equilibrium density centrifugation in concert with reverse transcription-PCR and denaturing gradient gel electrophoresis. In contradiction with findings from conventional methodologies, this unique approach revealed that phenol degradation in the microbial community under investigation is dominated by a member of the
Thauera
genus. Our results suggest that this organism is important for the function of this bioreactor.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference35 articles.
1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
2. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
3. Anders, H.-J., A. Kaetzke, P. Kampfer, W. Ludwig, and G. Fuchs. 1995. Taxonomic position of aromatic-degrading denitrifying Pseudomonad strains K172 and KB740 and their description as new members of the genera Thauera, as Thauera aromatica sp. nov., and Azoarcus, as Azoarcus evansii sp. nov., respectively, members of the beta subclass of the Proteobacteria. Intl. J. Syst. Bacteriol.45:327-333.
4. Wide Distribution and Diversity of Members of the Bacterial Kingdom
Acidobacterium
in the Environment
5. Birnie G. D. 1978. Centrifugal methods for characterising macromolecules and their interactions p. 95-125. In G. D. Birnie and D. Rickwood (ed.) Centrifugal separations in molecular and cell biology. Butterworths London United Kingdom.
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