Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

Author:

Lee Jayeon12345,Jeon Chang Hoon12345,Ahn Sang Jung6789,Ha Tai Hwan12345

Affiliation:

1. Nanobiotechnology (Major)

2. School of Engineering

3. University of Science & Technology Centre

4. Daejeon 305-806, Republic of Korea

5. Research center of integrative cellolomics

6. Center for Nano-Imaging Technology

7. Division of Industrial Metrology

8. Korea Research Institute of Standards and Science (KRISS)

9. Daejeon 305-340, Republic of Korea

Abstract

Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5′-end.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference26 articles.

1. Ochratoxin A, a Toxic Metabolite produced by Aspergillus ochraceus Wilh.

2. FAO/WHO , Safety evaluation of certain mycotoxins in food. In prepared by 56th meeting of the joint FAO/WHO expert committee on food additives (JECFA) , International Programme on Chemical Safety , World Health Organization , 2001 , pp. 281–415

3. Kinetic parameters and intraindividual fluctuations of ochratoxin A plasma levels in humans

4. Effect of roasting on ochratoxin A level in green coffee beans inoculated with Aspergillus ochraceus

5. Thermostability of Ochratoxin A in wheat under two moisture conditions

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