Polymer-based microfluidic device for on-chip counter-diffusive crystallization and in situ X-ray crystallography at room temperature

Author:

Saha Sarthak1ORCID,Özden Can2ORCID,Samkutty Alfred2,Russi Silvia3ORCID,Cohen Aina3ORCID,Stratton Margaret M.2ORCID,Perry Sarah L.1ORCID

Affiliation:

1. Department of Chemical Engineering, University of Massachusetts Amherst, MA 01003, USA

2. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, MA 01003, USA

3. SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA

Abstract

Here we have reported novel polymeric devices to control fluid flow using surface forces and capillary action to facilitate counter-diffusive, on-chip protein crystallization.

Funder

National Institutes of Health

PPG Industries

National Science Foundation

Basic Energy Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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