HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
Author:
Affiliation:
1. Department of Mechanical Engineering
2. University of Washington
3. Seattle
4. USA
5. Department of Chemical Engineering
6. PATH
Abstract
An HIV detection assay featuring a low-cost μPAD for electrophoretic RNA extraction and rapid isothermal nucleic acid amplification.
Funder
National Institute of Biomedical Imaging and Bioengineering
National Science Foundation
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2021/AN/D0AN02483J
Reference60 articles.
1. WHO | Data and statistics, http://www.who.int/hiv/data/en/
2. World Health Organization (WHO), Guideline on when to start antiretroviral therapy and on pre-exposure prophylaxis for HIV, http://apps.who.int/iris/bitstream/10665/186275/1/9789241509565_eng.pdf
3. World Health Organization , WHO recommendations on the diagnosis of HIV infection in infants and children , World Health Organization, and Department of HIV/AIDS , 2010
4. Scale-up of Routine Viral Load Testing in Resource-Poor Settings: Current and Future Implementation Challenges: Table 1.
5. Point-of-Care HIV Viral Load Testing: an Essential Tool for a Sustainable Global HIV/AIDS Response
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