SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control-Reference-Cited by-同舟云学术

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SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control

Published:2024 Issue:3 Volume:3 Page:421-430
ISSN:2635-0998
Container-title:Sensors & Diagnostics
language:en
Short-container-title:Sens. Diagn.

Author:

Martin Coleman D.¹^ORCID,Bender Andrew T.²^ORCID,Sullivan Benjamin P.²^ORCID,Lillis Lorraine³,Boyle David S.³,Posner Jonathan D.¹²⁴^ORCID

Affiliation:

1. Department of Chemical Engineering, University of Washington, Seattle, Washington, USA

2. Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA

3. PATH, Seattle, Washington, USA

4. Department of Family Medicine, University of Washington, Seattle, Washington, USA

Abstract

Here we report on a SARS-CoV-2 RPA assay for lateral flow strip detection format that incorporates a full process internal control that indicates the successful lysis, reverse transcription, and amplification of each viral sample.

Funder

National Center for Advancing Translational Sciences

M.J. Murdock Charitable Trust

National Institute of Biomedical Imaging and Bioengineering

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SD/D3SD00246B

Reference43 articles.

1. The coronavirus is here to stay — here’s what that means

2. Diagnostics for COVID-19: moving from pandemic response to control

3. SARS-CoV-2 viral load and shedding kinetics

4. Diagnostic Performance of an Antigen Test with RT-PCR for the Detection of SARS-CoV-2 in a Hospital Setting — Los Angeles County, California, June–August 2020

5. Rapid Diagnostic Testing for SARS-CoV-2

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