Cu(<scp>ii</scp>)-based DNA labeling identifies the structural link between transcriptional activation and termination in a metalloregulator-Reference-Cited by-同舟云学术

Cu(ii)-based DNA labeling identifies the structural link between transcriptional activation and termination in a metalloregulator

Published:2022 Issue:6 Volume:13 Page:1693-1697
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Casto Joshua¹^ORCID,Mandato Alysia¹,Hofmann Lukas²,Yakobov Idan²,Ghosh Shreya¹^ORCID,Ruthstein Sharon²^ORCID,Saxena Sunil¹^ORCID

Affiliation:

1. Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA

2. Department of Chemistry, Faculty of Exact Sciences, The Institution of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, Israel

Abstract

Herein, we exploit site-directed Cu(ii)-labeling to measure the DNA conformations in each step of the transcription cycle of the Copper Efflux Regulator (CueR), in order to establish how transcription is activated and terminated.

Funder

National Science Foundation

United States - Israel Binational Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D1SC06563G

Reference37 articles.

1. Metal sensor proteins: nature's metalloregulated allosteric switches

2. Mechanisms of copper homeostasis in bacteria