Affiliation:
1. Department of Chemistry and the Institute of Nanotechnology and Advanced Materials Bar-Ilan University Ramat-Gan Israel 5290002.
2. Department of Chemistry University of Pittsburgh Pittsburgh PA 15260
Abstract
AbstractBacteria use specialized proteins, like transcription factors, to rapidly control metal ion balance. CueR is a Gram‐negative bacterial copper regulator. The structure of E. coli CueR complexed with Cu(I) and DNA was published, since then many studies have shed light on its function. However, P. aeruginosa CueR, which shows high sequence similarity to E. coli CueR, has been less studied. Here, we applied room‐temperature electron paramagnetic resonance (EPR) measurements to explore changes in dynamics of P. aeruginosa CueR in dependency of copper concentrations and interaction with two different DNA promoter regions. We showed that P. aeruginosa CueR is less dynamic than the E. coli CueR protein and exhibits much higher sensitivity to DNA binding as compared to its E. coli CueR homolog. Moreover, a difference in dynamical behavior was observed when P. aeruginosa CueR binds to the copZ2 DNA promoter sequence compared to the mexPQ‐opmE promoter sequence. Such dynamical differences may affect the expression levels of CopZ2 and MexPQ‐OpmE proteins in P. aeruginosa. Overall, such comparative measurements of protein‐DNA complexes derived from different bacterial systems reveal insights about how structural and dynamical differences between two highly homologous proteins lead to quite different DNA sequence‐recognition and mechanistic properties.