Directed evolution of an alcohol dehydrogenase for the desymmetric reduction of 2,2-disubstituted cyclopenta-1,3-diones by enzymatic hydrogen transfer-Reference-Cited by-同舟云学术

Directed evolution of an alcohol dehydrogenase for the desymmetric reduction of 2,2-disubstituted cyclopenta-1,3-diones by enzymatic hydrogen transfer

Published:2022 Issue:19 Volume:12 Page:5841-5849
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Zhang Hongliu¹,Zhu Liangyan¹²,Feng Jinhui¹^ORCID,Liu Xiangtao¹,Chen Xi¹²^ORCID,Wu Qiaqing¹^ORCID,Zhu Dunming¹²^ORCID

Affiliation:

1. Tianjin Engineering Research Center of Biocatalytic Technology, National Engineering Research Center of Industrial Enzymes and National Technology Innovation Center of Synthetic Biology, Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences, Tianjin Airport Economic Area, 32 Xi Qi Dao, Tianjin 300308, P. R. China

2. University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Shijingshan District, Beijing, 100049 P.R. China

Abstract

Directed evolution of carbonyl reductase TbADH created mutant Tb2 with balanced activity toward ethyl secodione and isopropanol, enabling the desymmetric reduction of ethyl secodione to give (13R,17S)-ethyl secol with 94% yield, >99% ee and >99% de.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CY/D2CY00559J

Reference38 articles.

1. Totalsynthese optisch aktiver Steroide, I. Mikrobiologische stereospezifische Reduktion von 3-Methoxy-8.14-seco-1.3.5(10).9-östratetraen-14.17-dion

2. An expeditious asymmetric synthesis of the pentacyclic core of the cortistatins by an intramolecular (4+3) cycloaddition