A dual-mode strategy based on β-galactosidase and target-induced DNA polymerase protection for transcription factor detection using colorimetry and a glucose meter-Reference-Cited by-同舟云学术

A dual-mode strategy based on β-galactosidase and target-induced DNA polymerase protection for transcription factor detection using colorimetry and a glucose meter

Published:2023 Issue:23 Volume:148 Page:6078-6086
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Qian Xinmei¹²,Zhang Heng²,Zheng Mingyu¹,Li Chunxiang²,Wang Jinglun²,Huang Haowen¹,Deng Keqin¹^ORCID

Affiliation:

1. Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China

2. Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China

Abstract

β-Galactosidase was utilized for dual-signal transduction and the HCR-CRISPR/Cas12a system was used for cascade signal amplification. A dual-mode strategy was realized using a glucose meter and colorimetry for transcription factor detection.

Funder

Henan Province Science and Technology Innovation Talent Program

Natural Science Foundation ofHunan Province

Education Department of Hunan Province

Ministry of Education of the People's Republic of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN01414B

Reference47 articles.

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