Determining transaminase activity in bacterial libraries by time-lapse imaging-Reference-Cited by-同舟云学术

Determining transaminase activity in bacterial libraries by time-lapse imaging

Published:2019 Issue:90 Volume:55 Page:13538-13541
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Rodrigues Carlos J. C.¹²³⁴⁵^ORCID,Sanches João M.⁶⁷³⁴⁵^ORCID,de Carvalho Carla C. C. R.¹²³⁴⁵^ORCID

Affiliation:

1. iBB-Institute for Bioengineering and Biosciences

2. Department of Bioengineering

3. Instituto Superior Técnico

4. Universidade de Lisboa

5. Lisbon

6. Institute for Systems and Robotics (ISR/IST)

7. LARSyS, Bioengineering Department

Abstract

Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. The correlation between substrate concentration and luminance allows the screening of biocatalysts and determination of kinetic parameters.

Funder

Fundação para a Ciência e a Tecnologia

Horizon 2020 Framework Programme

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2019/CC/C9CC07507K

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2. Industrial applications of enzyme biocatalysis: Current status and future aspects

3. Whole cell biocatalysts: essential workers from Nature to the industry

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