The positional and numerical effect of <i>N</i><sup>6</sup>-methyladenosine in tracrRNA on the DNA cleavage activity of Cas9-Reference-Cited by-同舟云学术

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The positional and numerical effect of N⁶-methyladenosine in tracrRNA on the DNA cleavage activity of Cas9

Published:2024 Issue:29 Volume:14 Page:20529-20535
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Tran Binh Thanh¹,Go Seulgi¹,Kim Kyoung-Ran¹,Chung Hak Suk¹²^ORCID,Ahn Dae-Ro¹²^ORCID

Affiliation:

1. Chemical and Biological Integrative Research Center, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea

2. Division of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of Korea

Abstract

The m6A-modified tracrRNA can retain Cas9-mediated DNA cleavage activity only when the modified residues are located in the tail part of the tracrRNA, which is composed of stem loops 2 and 3.

Funder

Korea Institute of Science and Technology

National Research Foundation of Korea

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/RA/D4RA03957B

Reference22 articles.

1. The new frontier of genome engineering with CRISPR-Cas9

2. Development and Applications of CRISPR-Cas9 for Genome Engineering

3. A guide to genome engineering with programmable nucleases

4. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

5. Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines

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