Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells-Reference-Cited by-同舟云学术

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Published:2015-09 Issue:9 Volume:33 Page:985-989
ISSN:1087-0156
Container-title:Nature Biotechnology
language:en
Short-container-title:Nat Biotechnol

Author:

Hendel Ayal,Bak Rasmus O,Clark Joseph T,Kennedy Andrew B,Ryan Daniel E,Roy Subhadeep,Steinfeld Israel,Lunstad Benjamin D,Kaiser Robert J,Wilkens Alec B,Bacchetta Rosa,Tsalenko Anya,Dellinger Douglas,Bruhn Laurakay,Porteus Matthew H

Publisher

Springer Science and Business Media LLC

Subject

Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://www.nature.com/articles/nbt.3290.pdf

Reference24 articles.

1. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).

3. Cong, L. et al. Multiplex genome engineering using CRISPR-Cas systems. Science 339, 819–823 (2013).

4. Hsu, P.D., Lander, E.S. & Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262–1278 (2014).

5. Hendel, A., Fine, E.J., Bao, G. & Porteus, M.H. Quantifying on- and off-target genome editing. Trends Biotechnol. 33, 132–140 (2015).

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