Micropropagation and cryopreservation of the rare endemic Colchicum figlalii germplasm

Author:

Kaya Ergun1,Koyuncu Onur2,Şimşek Özhan3,Çürük Pembe Evci4,Mendi Yeşim Yalçın5

Affiliation:

1. Muğla S??tk?? Ko??man University, Faculty of Science, Molecular Biology and Genetics Department, 48000, Muğla, Turkey

2. Eskişehir Osmangazi Univerity, Special Education Application and Research Center, 26040 Odunpazar??-Eski??ehir, Turkey

3. Erciyes University, Seyrani Faculty of Agriculture, Department of Horticulture, 38030, MelikgaziKayseri, Turkey

4. Osmaniye Korkut Ata University, Kadirli School of Applied Sciences, 80760, Kadirli-Osmaniye, Turkey

5. Çukurova University, Faculty of Agriculture, Department of Horticulture, 01250 Sar????am-Adana, Turkey

Abstract

BACKGROUND: The natural population of Colchicum figlalii (Varol) Parolly & Eren grows in a narrow area of serpentine rock clearings at an altitude of 1900-2100 m in Southwestern Anatolia (Sandras Mountain, Mugla, Turkey). The species is regarded as endangered according to the IUCN Red List Categories. OBJECTIVE: To develop an optimum procedure for in vitro propagation and cryopreservation of germplasm of this rare endemic. MATERIALS AND METHODS: A total of 281 bulbs were used as in vitro culture starting material and after surface sterilization, clean material was obtained from 157 of them. Woody Plant Medium (WPM), Olive Medium (OM), and Murashige and Skoog medium (MS) were used for in vitro culture establishment. RESULTS: The maximum regeneration rate (˜67.3%) was obtained after four weeks of incubation on OM. The calli were successfully induced by using OM supplemented with 10.7 μM NAA from leaves of in vitro grown C. figlalii bulbs. A PVS2-vitrification procedure was used for cryopreservation of C. figlalii callus tissue. After cryo-storage, the best result for regeneration (66.7%) was obtained from calli treated with PVS2 for 75 min before plunging into liquid nitrogen. All rooted seedlings derived from cryopreserved calli were successfully acclimatized to greenhouse conditions. CONCLUSION: This study is an effective reference for future long-term conservation of similar species that are difficult to cryopreserve.

Publisher

CryoLetters Limited Liability Partnership

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3