Comparison of charantin extract from Momordica Charantia using modified supercritical carbon dioxide and soxhlet extraction method

Abstract

Charantin extract from Momordica Charantia fruits for anti-diabetic compound was proposed via Supercritical Fluid Extraction using carbon dioxide (SC-CO2) as solvent. This method is a promising alternative technique to a conventional Soxhlet and shaking water bath extraction method. Experiments were conducted to analyze the charantin i) with and without modifier (ethanol), ii) Soxhlet extraction with four types of solvent (distilled water, ethanol, dichloromethane, and petroleum ether) and iii) shaking water bath with distilled water as a solvent. A purification step was conducted post extraction to remove intrusive components such as chlorophylls and sugar from analyte in order to quantify charantin extract using high performance liquid chromatography (HPLC). The results show that, in 2.5 hours SC-CO2 with ethanol as modifier gives highest charantin yield with 0.7817 mg charantin per gram sample. In contrast, shaking water bath gives the highest charantin yield for conventional method with 0.712 mg charantin per gram sample during 6 hours of extraction. The result shows that by using SC-CO2 modified with ethanol can be a promising “green” extraction than conventional method.