Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies
Author:
Publisher
Springer Science and Business Media LLC
Subject
Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
http://www.nature.com/articles/nbt1097.pdf
Reference30 articles.
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3. Otomo, T., Ito, N., Kyogoku, Y. & Yamazaki, T. NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. Biochemistry 38, 16040–16044 (1999).
4. McIntosh, L.P. & Dahlquist, F.W. Biosynthetic incorporation of 15N and 13C for assignment and interpretation of nuclear magnetic resonance spectra of proteins. Q. Rev. Biophys. 23, 1–38 (1990).
5. Pervushin, K., Riek, R., Wider, G. & Wüthrich, K. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc. Natl. Acad. Sci. USA 94, 12366–12371 (1997).
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