Affiliation:
1. Department of Chemistry and Chemistry Institute for Functional Materials Pusan National University Busan 46241 Republic of Korea
2. Department of Chemistry and Integrative Institute of Basic Science Soongsil University Seoul 06978 Republic of Korea
Abstract
AbstractSplit systems, modular entities enabling controlled biological processes, have become instrumental in biological research. This review highlights their utility across applications like gene regulation, protein interaction identification, and biosensor development. Covering significant progress over the last decade, it revisits traditional split proteins such as GFP, luciferase, and inteins, and explores advancements in technologies like Cas proteins and base editors. We also examine reassembly modules and their applications in diverse fields, from gene regulation to therapeutic innovation. This review offers a comprehensive perspective on the recent evolution of split systems in biological research.
Funder
Pusan National University