Detection of protein-protein interactions using the GST fusion protein pull-down technique
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry,Biotechnology
Link
http://www.nature.com/articles/nmeth1204-275.pdf
Reference3 articles.
1. Smith, D.B. & Johnson, K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions of glutathione-S-transferase. Gene 67, 31–40 (1988).
2. Kaelin, W.G. Jr, Pallas, D.C., DeCaprio, J.A., Kaye, F.J. & Livingston, D.M. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell 64, 521–532 (1991).
3. Orlinick, J.R. & Chao, M.V. Interactions of the cellular polypeptides with the cytoplasmic domain of the mouse Fas antigen. J. Biol. Chem. 271, 8627–8632 (1996).
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