Fusion PCR and gene targeting in Aspergillus nidulans-Reference-Cited by-同舟云学术

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Fusion PCR and gene targeting in Aspergillus nidulans

Published:2006-12 Issue:6 Volume:1 Page:3111-3120
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Szewczyk Edyta,Nayak Tania,Oakley C Elizabeth,Edgerton Heather,Xiong Yi,Taheri-Talesh Naimeh,Osmani Stephen A,Oakley Berl R

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2006.405.pdf

Reference14 articles.

1. Dunne, P.W. & Oakley, B.R. Mitotic gene conversion, reciprocal recombination and gene replacement at the benA, β-tubulin locus of Aspergillus nidulans. Mol. Gen. Genet. 213, 339–345 (1988).

2. Toews, M.W. et al. Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (Gateway). Curr. Genet. 45, 383–389 (2004).

3. Kuwayama, H. et al. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acids Res. 30, E2 (2002).

4. Yang, L. et al. Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryotic Cell 3, 1359–1362 (2004).

5. Yu, J.-H. et al. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41, 973–981 (2004).

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