Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS-Reference-Cited by-同舟云学术

Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS

Published:2006-12 Issue:6 Volume:1 Page:2784-2790
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Blonder Josip,Chan King C,Issaq Haleem J,Veenstra Timothy D

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2006.359.pdf

Reference27 articles.

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2. Hopkins, A.L. & Groom, C.R. The druggable genome. Nat. Rev. Drug Discov. 1, 727–730 (2002).

3. Wu, C.C. & Yates, J.R. The application of mass spectrometry to membrane proteomics. Nat. Biotechnol. 21, 262–267 (2003).

4. Aebersold, R. & Mann, M. Mass spectrometry-based proteomics. Nature 422, 198–207 (2003).

5. Santoni, V., Molloy, M. & Rabilloud, T. Membrane proteins and proteomics: un amour impossible? Electrophoresis 21, 1054–1070 (2000).

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