Abstract
AbstractWhile algal phago-mixotrophs play a major role in aquatic microbial food webs, their diversity remains poorly understood. Recent studies have indicated several species of prasinophytes, early diverging green algae, to be able to consume bacteria for nutrition. To further explore the occurrence of phago-mixotrophy in green algae, we conducted feeding experiments with live fluorescently labeled bacteria stained with CellTracker Green CMFDA, heat-killed bacteria stained with 5-(4,6-dichlorotriazin-2-yl) aminofluorescein (DTAF), and magnetic beads. Feeding was detected via microscopy and/or flow cytometry in five strains of prasinophytes when provided with live bacteria: Pterosperma cristatum NIES626, Pyramimonas parkeae CCMP726, Pyramimonas parkeae NIES254, Nephroselmis pyriformis RCC618, and Dolichomastix tenuilepis CCMP3274. No feeding was detected when heat-killed bacteria or magnetic beads were provided, suggesting a strong preference for live prey in the strains tested. In parallel to experimental assays, green algal bacterivory was investigated using a gene-based prediction model. The predictions agreed with the experimental results and suggested bacterivory potential in additional green algae. Our observations underline the likelihood of widespread occurrence of phago-mixotrophy among green algae, while additionally highlighting potential biases introduced when using prey proxy to evaluate bacterial ingestion by algal cells.
Funder
National Science Foundation
Simons Foundation
Publisher
Springer Science and Business Media LLC
Subject
Ecology, Evolution, Behavior and Systematics,Microbiology
Reference93 articles.
1. Jost C, Lawrence CA, Campolongo F, Van De Bund W, Hill S, DeAngelis DL. The effects of mixotrophy on the stability and dynamics of a simple planktonic food web model. Theor Popul Biol. 2004;66:37–51.
2. Tittel J, Bissinger V, Zippel B, Gaedke U, Bell E, Lorke A, et al. Mixotrophs combine resource use to outcompete specialists: Implications for aquatic food webs. Proc Natl Acad Sci. 2011;100:12776–81.
3. Ward BA, Follows MJ. Marine mixotrophy increases trophic transfer efficiency, mean organism size, and vertical carbon flux. Proc Natl Acad Sci. 2016;113:2958–63.
4. Hansen PJ, Tillmann U. Mixotrophy among dinoflagellates—prey selection, physiology and ecological imporance. In: Subba Rao DV, editor. Dinoflagellates: classification, evolution, physiology and ecological significance. Hauppauge, NY, USA: Nova; 2020;201–60.
5. Unrein F, Gasol JM, Not F, Forn I, Massana R. Mixotrophic haptophytes are key bacterial grazers in oligotrophic coastal waters. ISME J. 2014;8:164–76.
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