A haemagglutination test for rapid detection of antibodies to SARS-CoV-2
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Published:2021-03-29
Issue:1
Volume:12
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Townsend AlainORCID, Rijal Pramila, Xiao Julie, Tan Tiong KitORCID, Huang Kuan-Ying A.ORCID, Schimanski Lisa, Huo Jiandong, Gupta NimeshORCID, Rahikainen RolleORCID, Matthews Philippa C., Crook Derrick, Hoosdally Sarah, Dunachie SusannaORCID, Barnes EleanorORCID, Street Teresa, Conlon Christopher P.ORCID, Frater JohnORCID, Arancibia-Cárcamo Carolina V., Rudkin Justine, Stoesser NicoleORCID, Karpe Fredrik, Neville MatthewORCID, Ploeg RutgerORCID, Oliveira Marta, Roberts David J., Lamikanra Abigail A., Tsang Hoi Pat, Bown Abbie, Vipond Richard, Mentzer Alexander J.ORCID, Knight Julian C.ORCID, Kwok Andrew J.ORCID, Screaton Gavin R.ORCID, Mongkolsapaya JuthathipORCID, Dejnirattisai Wanwisa, Supasa Piyada, Klenerman Paul, Dold Christina, Baillie J. KennethORCID, Moore Shona C.ORCID, Openshaw Peter J. M.ORCID, Semple Malcolm G.ORCID, Turtle Lance C. W.ORCID, Ainsworth Mark, Allcock Alice, Beer SallyORCID, Bibi Sagida, Skelly DonalORCID, Stafford Lizzy, Jeffrey KatieORCID, O’Donnell Denise, Clutterbuck Elizabeth, Espinosa Alexis, Mendoza Maria, Georgiou Dominique, Lockett Teresa, Martinez Jose, Perez Elena, Gallardo Sanchez Veronica, Scozzafava Giuseppe, Sobrinodiaz Alberto, Thraves Hannah, Joly EtienneORCID
Abstract
AbstractSerological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.
Funder
The research of EJ was funded by a private donation
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Reference35 articles.
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