A genetically encoded fluorescent biosensor for extracellular l-lactate-Reference-Cited by-同舟云学术

A genetically encoded fluorescent biosensor for extracellular l-lactate

Published:2021-12 Issue:1 Volume:12 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Nasu Yusuke,Murphy-Royal Ciaran^ORCID,Wen Yurong,Haidey Jordan N.,Molina Rosana S.^ORCID,Aggarwal Abhi,Zhang Shuce^ORCID,Kamijo Yuki,Paquet Marie-Eve^ORCID,Podgorski Kaspar^ORCID,Drobizhev Mikhail^ORCID,Bains Jaideep S.,Lemieux M. Joanne^ORCID,Gordon Grant R.^ORCID,Campbell Robert E.^ORCID

Abstract

Abstractl-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of l-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular l-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular l-lactate in cultured mammalian cells and brain tissue.

Funder

MEXT | Japan Society for the Promotion of Science

Toyota Physical and Chemical Research Institute

Gouvernement du Canada | Canadian Institutes of Health Research

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

The Precise Measurement Technology Promotion Foundation

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

Link

https://www.nature.com/articles/s41467-021-27332-2.pdf

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