Mapping protein selectivity landscapes using multi-target selective screening and next-generation sequencing of combinatorial libraries-Reference-Cited by-同舟云学术

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Mapping protein selectivity landscapes using multi-target selective screening and next-generation sequencing of combinatorial libraries

Published:2018-09-26 Issue:1 Volume:9 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Naftaly Si,Cohen Itay,Shahar Anat,Hockla Alexandra,Radisky Evette S.,Papo Niv

Funder

EC | European Research Council

United States - Israel Binational Science Foundation

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

Link

http://www.nature.com/articles/s41467-018-06403-x.pdf

Reference74 articles.

1. Aizner, Y. et al. Mapping of the binding landscape for a picomolar protein-protein complex through computation and experiment. Structure 22, 636–645 (2014).

2. Gfeller, D. et al. The multiple-specificity landscape of modular peptide recognition domains. Mol. Syst. Biol. 7, 484 (2011).

3. Sharabi, O. et al. Affinity- and specificity-enhancing mutations are frequent in multispecific interactions between TIMP2 and MMPs. PLoS ONE 9, e93712 (2014).

4. Fowler, D. M. & Fields, S. Deep mutational scanning: a new style of protein science. Nat. Methods 11, 801–807 (2014).

5. Fowler, D. M., Stephany, J. J. & Fields, S. Measuring the activity of protein variants on a large scale using deep mutational scanning. Nat. Protoc. 9, 2267–2284 (2014).

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