Extending resolution within a single imaging frame

Author:

Torres-García EsleyORCID,Pinto-Cámara RaúlORCID,Linares Alejandro,Martínez DamiánORCID,Abonza Víctor,Brito-Alarcón EduardoORCID,Calcines-Cruz CarlosORCID,Valdés-Galindo Gustavo,Torres David,Jabloñski Martina,Torres-Martínez Héctor H.ORCID,Martínez José L.,Hernández Haydee O.ORCID,Ocelotl-Oviedo José P.,Garcés Yasel,Barchi MarcoORCID,D’Antuono RoccoORCID,Bošković Ana,Dubrovsky Joseph G.ORCID,Darszon Alberto,Buffone Mariano G.,Morales Roberto Rodríguez,Rendon-Mancha Juan Manuel,Wood Christopher D.ORCID,Hernández-García Armando,Krapf DiegoORCID,Crevenna Álvaro H.ORCID,Guerrero Adán

Abstract

AbstractThe resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.

Funder

Consejo Nacional de Ciencia y Tecnología

Ministry of Science, Technology and Productive Innovation, Argentina | Agencia Nacional de Promoción Científica y Tecnológica

Silicon Valley Community Foundation

National Science Foundation

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary

Cited by 24 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3