Super-resolution structured illumination microscopy: past, present and future

Author:

Prakash Kirti12ORCID,Diederich Benedict34ORCID,Reichelt Stefanie5ORCID,Heintzmann Rainer346ORCID,Schermelleh Lothar7

Affiliation:

1. National Physical Laboratory, TW11 0LW Teddington, UK

2. Department of Paediatrics, Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK

3. Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany

4. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, Jena, Germany

5. CRUK Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE, UK

6. Faculty of Physics and Astronomy, Friedrich-Schiller-University, Jena, Germany

7. Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK

Abstract

Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware and software development and the use of commercial instruments to biological applications. This special issue aims to recap recent developments as well as outline future trends. In addition to SIM, we cover related topics such as complementary super-resolution microscopy techniques, computational imaging, visualization and image processing methods. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

Publisher

The Royal Society

Subject

General Physics and Astronomy,General Engineering,General Mathematics

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