Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine
Author:
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,Molecular Medicine
Link
http://www.nature.com/articles/3300975.pdf
Reference14 articles.
1. Monaco AP, Larin Z . YACs, BACs, PACs and MACs: artificial chromosomes as research tools Trends Biotechnol 1994 12: 280–286
2. Larin Z, Monaco AP, Lehrach H . Yeast artificial chromosome libraries containing large inserts from mouse and human DNA Proc Natl Acad Sci USA 1991 88: 4123–4127
3. Montoliu L, Bock C-T, Schütz G, Zentgraf H . Visualization of large DNA molecules by electron microscopy with polyamines: application to the analysis of yeast endogenous chromosomes and artificial chromosomes J Mol Biol 1995 246: 486–849
4. Bauchwitz R, Constantini F . YAC transgenesis: a study of conditions to protect YAC DNA from breakage and a protocol for transfection Biochem Biophys Acta 1998 1401: 21–37
5. Lee JT, Jaenisch R . A method for high efficiency YAC lipofection into murine embryonic stem cells Nucleic Acids Res 1996 24: 5054–5055
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