Exogenous artificial DNA forms chromatin structure with active transcription in yeast
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Published:2021-12-27
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Volume:
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ISSN:1674-7305
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Container-title:Science China Life Sciences
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language:en
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Short-container-title:Sci. China Life Sci.
Author:
Zhou Jianting,Zhang Chao,Wei Ran,Han Mingzhe,Wang Songduo,Yang Kaiguang,Zhang Lihua,Chen Weigang,Wen Mingzhang,Li Cheng,Tao Wei,Yuan Ying-Jin
Abstract
AbstractYeast artificial chromosomes (YACs) are important tools for sequencing, gene cloning, and transferring large quantities of genetic information. However, the structure and activity of YAC chromatin, as well as the unintended impacts of introducing foreign DNA sequences on DNA-associated biochemical events, have not been widely explored. Here, we showed that abundant genetic elements like TATA box and transcription factor-binding motifs occurred unintentionally in a previously reported data-carrying chromosome (dChr). In addition, we used state-of-the-art sequencing technologies to comprehensively profile the genetic, epigenetic, transcriptional, and proteomic characteristics of the exogenous dChr. We found that the data-carrying DNA formed active chromatin with high chromatin accessibility and H3K4 tri-methylation levels. The dChr also displayed highly pervasive transcriptional ability and transcribed hundreds of noncoding RNAs. The results demonstrated that exogenous artificial chromosomes formed chromatin structures and did not remain as naked or loose plasmids. A better understanding of the YAC chromatin nature will improve our ability to design better data-storage chromosomes.
Publisher
Springer Science and Business Media LLC
Subject
General Agricultural and Biological Sciences,General Environmental Science,General Biochemistry, Genetics and Molecular Biology
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