Abstract
AbstractBed bug has become a major public health pest worldwide. Infestation may result in numerous negative health effects. Homeless shelters are one of the most habitats that can be infested with bed bugs, a few studies have focused on bed bug infestations in these settings. We conducted a survey of infestations of bed bugs in a homeless shelter in southern France, using an innovative seven-level scale (0–6) to assess the degree of infestation, MALDI TOF-MS to identify bed bugs, and a biomolecular tool to detect bacteria. Bed bug infestations were documented in 13% (9/68) of investigated rooms. A total of 184 bed bugs were collected and morphologically identified as Cimex lectularius. MALDI TOF–MS analysis allowed us to obtain high-quality MS spectra for all 184 specimens, to correctly identify all specimens, and included 178/184 (97%) Log Score Values higher than 1.8. Among the bacteria tested, Wolbachia sp. DNA was found in 149/184 (81%) of the bed bugs, and one sample was positive for Coxiella burnetii, the agent of Q fever. Our study is the first of its kind that offers new perspectives for increasing public awareness of the conditions in homeless shelters.
Funder
Institut Hospitalo-Universitaire (IHU) Méditerranée
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. Gangloff-Kaufmann, J. & Pichler, C. Guidelines for prevention and management of bed bugs in shelters and group living facilities. (2008).
2. Doggett, S. L., Dwyer, D. E., Peñas, P. F. & Russell, R. C. Bed bugs: Clinical relevance and control options. Clin. Microbiol. Rev. 25, 164–192 (2012).
3. Parola, P. & Izri, A. Bedbugs. N. Engl. J. Med. 382, 2230–2237 (2020).
4. Delaunay, P. et al. Bedbugs and infectious diseases. Clin. Infect. Dis. 52, 200–210 (2011).
5. Benkacimi, L. et al. MALDI-TOF MS identification of Cimex lectularius and Cimex hemipterus bedbugs. Infect. Genet. Evol. 85, 104536 (2020).
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