Investigation of Hepatitis B Virus in the Body of the Bed Bug Cimex hemipterus (Hemiptera: Cimicidae) Fed on Infected Human Blood by RT-PCR Method

Abstract

Background Bed bugs (Hemiptera: Cimicidae) are insects found in abundance among urban and rural communities. Both male and female bed bugs thrive on human blood. The main aim of this study was to detect Hepatitis B Virus (HBV) in the body of the bed bug, Cimex hemipterus (Hemiptera: Cimicidae), fed on naturally infected patient blood by Reverse Transcription Polymerase Chain Reaction (RT-PCR) Methods In this experiment, C. hemipterus bugs nourished once with HBV-positive blood were examined by RT-PCR at 1, 2, 3, 7- and 10 days post-engorgement. Bloodsucking was performed using an artificial membrane feeding system. RNA was extracted from infected bed bugs, and cDNA was then synthesized to monitor the HBV mRNA. Results The RT-PCR test results for infected specimens were positive on the first to third days post-bloodsucking. Moreover, the sample on days 7-10 was negative for HBV. This study also revealed that the breeding of bed bugs under laboratory conditions lasted for one week from the first to fifth nymph stages. Conclusion Since HBV mRNA was detected in infected bed bugs until the third-day post-engorgement, it is likely that they potentially transmitted HBV. It is thus indispensable to conduct more thorough research in the future.