Author:
Kim Jun-Kyu,Youn Young-Jin,Lee Yu-Bin,Kim Sun-Hwa,Song Dong-Keun,Shin Minsang,Jin Hee Kyung,Bae Jae-sung,Shrestha Sanjeeb,Hong Chang-Won
Abstract
AbstractExtracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.
Funder
National Research Foundation of Korea
Publisher
Springer Science and Business Media LLC
Cited by
6 articles.
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