DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-019-46932-z.pdf
Reference32 articles.
1. Lafzi, A., Moutinho, C., Picelli, S. & Heyn, H. Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies. Nat Protoc 13, 2742–2757, https://doi.org/10.1038/s41596-018-0073-y (2018).
2. Chen, X., Teichmann, S. A. & Meyer, K. B. From Tissues to Cell Types and Back: Single-Cell Gene Expression Analysis of Tissue Architecture. Annual Review of Biomedical Data Science 1, 29–51, https://doi.org/10.1146/annurev-biodatasci-080917-013452 (2018).
3. Papalexi, E. & Satija, R. Single-cell RNA sequencing to explore immune cell heterogeneity. Nat Rev Immunol 18, 35–45, https://doi.org/10.1038/nri.2017.76 (2018).
4. Tang, F. et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods 6, 377–382, https://doi.org/10.1038/nmeth.1315 (2009).
5. Klein, A. M. et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161, 1187–1201, https://doi.org/10.1016/j.cell.2015.04.044 (2015).
Cited by 82 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics;2024-08-30
2. CryoSCAPE: Scalable Immune Profiling Using Cryopreserved Whole Blood for Multi-omic Single Cell and Functional Assays;2024-08-21
3. Simultaneous isolation of intact brain cells and cell-specific extracellular vesicles from cryopreserved Alzheimer’s disease cortex;Journal of Neuroscience Methods;2024-06
4. Cryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis;Journal of Neuroinflammation;2024-03-23
5. Infiltrative classical monocyte-derived andSPP1lipid-associated macrophages mediate inflammation and fibrosis in ANCA-associated glomerulonephritis;2024-03-13
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3