Author:
Samiksha ,Singh Drishtant,Kesavan Anup Kumar,Sohal Satwinder Kaur
Abstract
AbstractPeptidase inhibitors (PIs) are defense proteins of plants which are active against gut peptidases of different insects.Sapindus mukorossiwas identified as a source of bioactive PIs which could confer resistance againstBactrocera cucurbitae, a most devastating pest of several economically important crops. In the present study, a trypsin inhibitor was purified from mature dry seeds ofS. mukorossiand characterized for its biochemical properties as well as its potential for bio control ofB. cucurbitae. The purified fractions from RP- HPLC through SDS-PAGE gave an apparent molecular weight of ~29 kDa.S. mukorossitrypsin inhibitor (SMTI) was found to be a non-competitive inhibitor which was active over a broad range of temperature (10–100 °C) and pH (6–11). SMTI when incorporated in artificial diet inhibited the growth and development ofB. cucurbitaelarvae. Gene expression analysis of trypsin and chymotrypsin genes via qRT-PCR indicated that their mRNA expression was down-regulated while that of other genes namely, Catalase, Elastase, Superoxide Dismutase, Glutathione –S-transferase and Alkaline Phosphatase was up regulated. SMTI also showed deleterious effects against different bacterial strains. The results of this study indicated thatS. mukorossitrypsin inhibitor has potential to be used as a bio control agent that can reduce the harm caused by melon fruit fly and other devastating pests.
Funder
University Grants Commission
UGC New Delhi under the scheme University with Potential for Excellence
Publisher
Springer Science and Business Media LLC
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