E. coli aggregation and impaired cell division after terahertz irradiation

Author:

Peltek Sergey,Meshcheryakova Irina,Kiseleva Elena,Oshchepkov Dmitry,Rozanov Alexei,Serdyukov Danil,Demidov Evgeniy,Vasiliev Gennady,Vinokurov Nikolay,Bryanskaya Alla,Bannikova Svetlana,Popik Vasiliy,Goryachkovskaya Tatyana

Abstract

AbstractIn this study we demonstrated that exposure of Escherichia coli (E. coli) to terahertz (THz) radiation resulted in a change in the activities of the tdcABCDEFGR and matA–F genes (signs of cell aggregation), gene yjjQ (signs of suppression of cell motility), dicABCF, FtsZ, and minCDE genes (signs of suppression of cell division), sfmACDHF genes (signs of adhesin synthesis), yjbEFGH and gfcA genes (signs of cell envelope stabilization). Moreover, THz radiation induced E. coli csg operon genes of amyloid biosynthesis. Electron microscopy revealed that the irradiated bacteria underwent increased aggregation; 20% of them formed bundle-like structures consisting of two to four pili clumped together. This could be the result of changes in the adhesive properties of the pili. We also found aberrations in cell wall structure in the middle part of the bacterial cell; these aberrations impaired the cell at the initial stages of division and resulted in accumulation of long rod-like cells. Overall, THz radiation was shown to have adverse effects on bacterial populations resulting in cells with abnormal morphology.

Funder

Ministry of Education and Science of the Russian Federation

Kurchatov Genomics Center IC&G

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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