Author:
Vigliaturo Ruggero,Jamnik Maja,Dražić Goran,Podobnik Marjetka,Žnidarič Magda Tušek,Ventura Giancarlo Della,Redhammer Günther J.,Žnidaršič Nada,Caserman Simon,Gieré Reto
Abstract
AbstractAmphibole asbestos is related to lung fibrosis and several types of lung tumors. The disease-triggering mechanisms still challenge our diagnostic capabilities and are still far from being fully understood. The literature focuses primarily on the role and formation of asbestos bodies in lung tissues, but there is a distinct lack of studies on amphibole particles that have been internalized by alveolar epithelial cells (AECs). These internalized particles may directly interact with the cell nucleus and the organelles, exerting a synergistic action with asbestos bodies (AB) from a different location. Here we document the near-atomic- to nano-scale transformations induced by, and taking place within, AECs of three distinct amphiboles (anthophyllite, grunerite, “amosite”) with different Fe-content and morphologic features. We show that: (i) an Fe-rich layer is formed on the internalized particles, (ii) particle grain boundaries are transformed abiotically by the internal chemical environment of AECs and/or by a biologically induced mineralization mechanism, (iii) the Fe-rich material produced on the particle surface does not contain large amounts of P, in stark contrast to extracellular ABs, and (iv) the iron in the Fe-rich layer is derived from the particle itself. Internalized particles and ABs follow two distinct formation mechanisms reaching different physicochemical end-states.
Funder
Rotary Foundation
Javna Agencija za Raziskovalno Dejavnost RS
Ministero dell’Istruzione, dell’Università e della Ricerca
National Institute of Environmental Health Sciences
Publisher
Springer Science and Business Media LLC
Reference72 articles.
1. Jablonski, R. P., Kim, S. J., Cheresh, P. & Kamp, D. W. Insights into mineral fibre-induced lung epithelial cell toxicity and pulmonary fibrosis. EMU Not. Mineral. 18, 447–500 (2017).
2. Bernstein, D. et al. Health risk of chrysotile revisited. Crit. Rev. Toxicol. 43(2), 154–183 (2013).
3. U.S. National Research Council. Asbestiform fibres: nonoccupational health risks. National Academy Press, Washington (1985)
4. Gualtieri, A. F. et al. New insights into the toxicity of mineral fibres: A combined in situ synchrotron μ-XRD and HR-TEM study of chrysotile, crocidolite, and erionite fibres found in the tissues of Sprague-Dawley rats. Toxicol. Lett. 274, 20–30 (2017).
5. Gaudino, G., Xue, J. & Yang, H. How asbestos and other fibers cause mesothelioma. Transl. Lung Cancer Res. 9(Suppl 1), S39–S46 (2020).
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献