HLA-A, -B, -C, -DRB1, -DQA1, and -DQB1 allele and haplotype frequencies defined by next generation sequencing in a population of East Croatia blood donors

Author:

Tokić Stana,Žižkova Veronika,Štefanić Mario,Glavaš-Obrovac Ljubica,Marczi Saška,Samardžija Marina,Sikorova Katerina,Petrek Martin

Abstract

AbstractNext-generation sequencing (NGS) is increasingly used in transplantation settings, but also as a method of choice for in-depth analysis of population-specific HLA genetic architecture and its linkage to various diseases. With respect to complex ethnic admixture characteristic for East Croatian population, we aimed to investigate class-I (HLA-A, -B, -C) and class-II (HLA-DRB1, -DQA1, -DQB1) HLA diversity at the highest, 4-field resolution level in 120 healthy, unrelated, blood donor volunteers. Genomic DNA was extracted and HLA genotypes of class I and DQA1 genes were defined in full-length, -DQB1 from intron 1 to 3′ UTR, and -DRB1 from intron 1 to intron 4 (Illumina MiSeq platform, Omixon Twin algorithms, IMGT/HLA release 3.30.0_5). Linkage disequilibrium statistics, Hardy-Weinberg departures, and haplotype frequencies were inferred by exact tests and iterative Expectation-Maximization algorithm using PyPop 0.7.0 and Arlequin v3.5.2.2 software. Our data provide first description of 4-field allele and haplotype frequencies in Croatian population, revealing 192 class-I and class-II alleles and extended haplotypic combinations not apparent from the existing 2-field HLA reports from Croatia. This established reference database complements current knowledge of HLA diversity and should prove useful in future population studies, transplantation settings, and disease-associated HLA screening.

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference58 articles.

1. Rendečić Miočević, D. Iliri i Antički Svijet. (The Illyrians and the Ancient World., ed. Mimica, I) Književni krug: Split, Croatia, 1–920 (1989).

2. Mujadžević, D. The other Ottoman serhat in Europe: Ottoman territorial expansion in Bosnia and Croatia in 16th century. Ankara Univ. J. Center South East Eur. Studies. 1, 99–111 (2012).

3. Pavičić, S. Slavonija u svojem naselnom razvitku od trinaestog stoljeća do danas. (Slavonia In Its Settlement Development From The Thirteenth Century To The Present., ed. V, Radauš) Zbornik radova I. znanstvenog sabora Slavonije i Baranje, Jugoslavenska akademija znanosti i umjetnosti, 191–236 (1970).

4. Sanchez-Mazas, A., Buhler, S. & Nunes, J. M. A new HLA map of Europe: Regional genetic variation and its implication for peopling history, disease-association studies and tissue transplantation. Hum. Hered. 76, 162–177 (2013).

5. Choo, S. Y. The HLA system: genetics, immunology, clinical testing, and clinical implications. Yonsei. Med. J. 48, 11–23 (2007).

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3