High-Resolution HLA-DRB1 Allele Frequencies in a Romanian Cohort of Stem Cell Donors-Reference-Cited by-同舟云学术

High-Resolution HLA-DRB1 Allele Frequencies in a Romanian Cohort of Stem Cell Donors

Published:2024-06-01 Issue:1 Volume:27 Page:43-49
ISSN:2199-5761
Container-title:Balkan Journal of Medical Genetics
language:en
Short-container-title:

Author:

Caragea MA¹²,Ursu IR³,Visan DL²,Maruntelu I¹²,Iordache P⁴,Constantinescu A¹,Tizu M¹²,Tălăngescu A¹²,Constantinescu I¹²

Affiliation:

1. Carol Davila University of Medicine and Pharmacy , Bucharest , Romania

2. Center for Immunogenetics and Virology , Fundeni Clinical Institute , Bucharest , Romania

3. Carol Davila University of Medicine and Pharmacy , Department of Medical Genetics , Bucharest , Romania

4. Carol Davila University of Medicine and Pharmacy , Department of Epidemiology , Bucharest , Romania

Abstract

Abstract The goal of the current study was to determine the high-resolution frequencies of the HLA-DRB1 alleles among the analyzed Romanian cohort of healthy stem cell donors. Using Next Generation Sequencing (NGS), we estimated class II HLA-DRB1 allele frequencies to a 6-digit resolution through HLA typing in a Romanian cohort of healthy individuals. The study for HLA genotyping included 420 willing donors from the National Registry of Voluntary Hematopoietic Stem Cell Donors (RNDVCSH). In 2020 and 2021, peripheral blood samples were collected and transported to the Fundeni Clinical Institute. We used the Immucor Mia Fora NGS MFlex kit for HLA genotyping. Forty-one different alleles were detected in 420 analyzed samples, out of which the most frequent HLA-DRB1 alleles were DRB1*16:01:01 (12.6%), DRB1*11:04:01 (12.1%) and DRB1*03:01:01 (12%). The HLA-DRB1*11:01:02 and -DRB1*08:04:01, -DRB1*05:01:01, -DRB1*13:05:01, -DRB1*14:07:01, -DRB1*09:01:02, -DRB1*11:02:01, -DRB1*04:07:01, -DRB1*15:03:01, -DRB1*03:02:01, -DRB1*04:06:02, -DRB1*04:08:01, -DRB1*14:05:01 were identified only once. The results revealed similarities with countries belonging to the Eastern Europe, the Balkans and the Caucasus regions. Further studies on larger Romanian cohorts are needed for confirming the current results.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/bjmg-2024-0009

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