Author:
Badaut Cyril,Visitdesotrakul Pimnitah,Chabry Aurélie,Bigey Pascal,Tornyigah Bernard,Roman Jocelyne,Maroufou Jules Alao,Amoussou Annick,Ayivi Blaise Serge,Sagbo Gratien,Ndam Nicaise Tuikue,Oleinikov Andrew V.,Tahar Rachida
Abstract
AbstractThe Plasmodium falciparum erythrocyte-membrane-protein-1 (PF3D7_1150400/PF11_0521) contains both domain cassette DC13 and DBLβ3 domain binding to EPCR and ICAM-1 receptors, respectively. This type of PfEMP1 proteins with dual binding specificity mediate specific interactions with brain micro-vessels endothelium leading to the development of cerebral malaria (CM). Using plasma collected from children at time of hospital admission and after 30 days, we study an acquisition of IgG response to PF3D7_1150400/PF11_0521 DC13 and DBLβ3_D4 recombinant constructs, and five peptides located within these constructs, specifically in DBLα1.7_D2 and DBLβ3_D4 domains. We found significant IgG responses against the entire DC13, PF11_0521_DBLβ3_D4 domain, and peptides. The responses varied against different peptides and depended on the clinical status of children. The response was stronger at day 30, and mostly did not differ between CM and uncomplicated malaria (UM) groups. Specifically, the DBLβ3 B3-34 peptide that contains essential residues involved in the interaction between PF11_0521 DBLβ3_D4 domain and ICAM-1 receptor demonstrated significant increase in reactivity to IgG1 and IgG3 antibodies at convalescence. Further, IgG reactivity in CM group at time of admission against functionally active (ICAM-1-binding) PF11_0521 DBLβ3_D4 domain was associated with protection against severe anemia. These results support development of vaccine based on the PF3D7_1150400/PF11_0521 structures to prevent CM.
Funder
National Institutes of Health
National French Agency of Research
Publisher
Springer Science and Business Media LLC
Reference40 articles.
1. MacPherson, G. G., Warrell, M. J., White, N. J., Looareesuwan, S. & Warrell, D. A. Human cerebral malaria. A quantitative ultrastructural analysis of parasitized erythrocyte sequestration. Am. J. Pathol. 119, 385–401 (1985).
2. Taylor, T. E. et al. Differentiating the pathologies of cerebral malaria by postmortem parasite counts. Nat. Med. 10, 143–145. https://doi.org/10.1038/nm986 (2004).
3. Potchen, M. J. et al. Acute brain MRI findings in 120 Malawian children with cerebral malaria: new insights into an ancient disease. AJNR Am. J. Neuroradiol. 33, 1740–1746. https://doi.org/10.3174/ajnr.A3035 (2012).
4. Seydel, K. B. et al. Brain swelling and death in children with cerebral malaria. N. Engl. J. Med. 372, 1126–1137. https://doi.org/10.1056/NEJMoa1400116 (2015).
5. Baruch, D. I. et al. Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes. Cell 82, 77–87 (1995).
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