Author:
Saleh Ahmed A.,Hafez Abdelhaleem,Amber Khairy,Abdelhady AbdelRahman Y.,Salem Heba M.,Fathy M.,Kamal M. A.,Alagawany Mahmoud,Alzawqari Mohammed H.
Abstract
AbstractThe study investigated the effect of enzymes as a toxin detoxifier (DETOXIZYME) dietary supplementation on performance during growth, blood chemistry, and immunity under clostridia infection in chickens. A total of 480, day-old male chicks were randomly distributed to four groups, with six replicates of 20 birds each. The first control negative treatment (A) fed the basal formula as commercial feed prepared following the strain's needs, the second control positive group (B) fed the basal formula challenged with Clostridium perfringens (C. perfringens) type A, the third group (C) fed the basal formula with 100 g DETOXIZYME/ton of feed and challenged with clostridia, and the fourth group (D) fed the control basal formula with 100 g DETOXIZYME/ton of feed. DETOXIZYME dietary supplementation significantly boosted body weight (BW), body weight gain (BWG), feed intake (FI), and European production efficiency factor (EPEF) and improved the feed conversion rate (FCR) of the broilers. The dietary supplementation of DETOXIZYME significantly increased carcass trait and spleen. However, liver and abdominal fat weight significantly decreased compared with clostridia-challenged groups. The values of alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid, creatinine, and Malondialdehyde (MDA) were decreased. While calcium, phosphate, zinc, and glutathione peroxidase (GPx) levels were improved in birds that took basal formulas fortified with DETOXIZYME contrary to the other treatment groups during 35 days of age. Plasma total cholesterol, triglyceride, and low-density lipoprotein (LDL) values were reduced versus the other treatment groups. Dietary supplementation of DETOXIZYME increased total protein, albumin, globulin, and Newcastle Disease (ND) immunity titer levels in the overall period compared to other groups. Dietary DETOXIZYME supplementation decreased clostridia and E. coli bacteria counts and improved gut morphometry. In conclusion, dietary supplementation of DETOXIZYME had a positive impact on performance, blood biochemistry, immunity, and bacterial counts and improved the gut morphology in broilers under clostridia infection.
Funder
The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank
Zagazig University
Publisher
Springer Science and Business Media LLC
Reference81 articles.
1. Abd El-Hack, M. E. et al. Alternatives to antibiotics for organic poultry production: Types, modes of action and impacts on bird’s health and production. Poult. Sci. 1, 101696 (2022).
2. Kamal, M. A., Khalaf, M. A., Zakia, A. M. A. & El-Jakee, J. K. Effect of water quality parameters on some health and reproductive indicators in cattle farms associated emerged epidemics in Egypt. Int. J. Vet. Sci. 8, 275–282 (2019).
3. Abd El-Hack, M. E. et al. The relationship among avian influenza, gut microbiota and chicken immunity: An updated overview. Poult. Sci. 1, 102021 (2022).
4. Kirrella, A. A. et al. Use of corn silk meal in broiler diet: Effect on growth performance, blood biochemistry, immunological responses, and growth-related gene expression. Animals 11, 1170 (2021).
5. Turner, J. R. Intestinal mucosal barrier function in health and disease. Nat. Rev. Immunol. 9, 799–809 (2009).