Author:
Li Hao,He Yujie,Lu Jinzhong,Jia Liling,Liu Yong,Yang Dan,Shao Shuai,Lv Gang,Yang Hailiang,Zheng Hailing,Zhou Yang,Peng Zhiqin
Abstract
AbstractHydrogen, oxygen, carbon, and nitrogen isotopes derived from three different strains of silkworms at different life stages involved in silkworm rearing, were measured to understand the fractionation characteristics of stable isotopes at different stages of silkworm development, and to trace the movement of these isotopes from food to larva to excrement and finally to silk. We found that silkworm strain had little effect on δ2H, δ18O and δ13C values. However, a large difference was found in the δ15N levels of newly-hatched silkworms between Jingsong Haoyue and Hua Kang No. 3 orthogonal strains, suggesting that the mating and egg laying differences may result in an inconsistent kinetic nitrogen isotope fractionation. The δ13C values of silkworm pupae and silkworm cocoon also displayed significant differences, suggesting that heavy carbon isotopes are greatly fractionated from the larva to the silk during cocoon formation. Overall, these results may be used to clarify the relationship between isotope fractionation and the ecological process of theBombyx moriand expand our ability to resolve stable isotope anomalies at a small regional-scale level.
Funder
National Key Research and Development Program of China
Zhejiang Provincial Natural Science Foundation of China
Zhejiang Provincial Administration of Cultural Heritage
Science and Technology Program of Gansu Province
Publisher
Springer Science and Business Media LLC