Author:
Fuzo Carlos Alessandro,da Veiga Ued Fábio,Moco Sofia,Cominetti Ornella,Métairon Sylviane,Pruvost Solenn,Charpagne Aline,Carayol Jerome,Torrieri Raul,Silva Wilson Araujo,Descombes Patrick,Kaput Jim,Monteiro Jacqueline Pontes
Abstract
AbstractPolymorphisms in genes related to the metabolism of vitamin B12 haven’t been examined in a Brazilian population.
To (a) determine the correlation between the local genetic ancestry components and vitamin B12 levels using ninety B12-related genes; (b) determine associations between these genes and their SNPs with vitamin B12 levels; (c) determine a polygenic risk score (PRS) using significant variants. This cross-sectional study included 168 children and adolescents, aged 9–13 years old. Total cobalamin was measured in plasma. Genotyping arrays and whole exome data were combined to yield ~ 7000 SNPs in 90 genes related to vitamin B12. The Efficient Local Ancestry Inference was used to estimate local ancestry for African (AFR), Native American, and European (EUR). The association between the genotypes and vitamin B12 levels were determined with generalized estimating equation.
Vitamin B12 levels were driven by positive (EUR) and negative (AFR, AMR) correlations with genetic ancestry. A set of 36 variants were used to create a PRS that explained 42% of vitamin level variation.
Vitamin B12 levels are influenced by genetic ancestry and a PRS explained almost 50% of the variation in plasma cobalamin in Brazilian children and adolescents.
Funder
Nestlé Research Lausanne Switzerland
Fundação de Amparo à Pesquisa do Estado de São Paulo
Publisher
Springer Science and Business Media LLC
Reference83 articles.
1. Pavlovic, M., Prentice, A., Thorsdottir, I., Wolfram, G. & Branca, F. Challenges in harmonizing energy and nutrient recommendations in Europe. Ann. Nutr. Metab. 51, 108–114 (2007).
2. Institute of Medicine. The Development of DRIs 1994–2004: Lessons Learned and New Challenges: Workshop Summary (National Academies Press, 2008).
3. Committee, D. G. A. Scientific Report of the 2020 Dietary Guidelines Advisory Committee Advisory Report to the Secretary of Agriculture and Secretary of Health and Human Services (U.S. Department of Agriculture, 2020).
4. Monteiro, J. P., Kussmann, M. & Kaput, J. The genomics of micronutrient requirements. Genes Nutr. 10, 466 (2015).
5. Van Ommen, B. et al. The Micronutrient Genomics Project: A community-driven knowledge base for micronutrient research. Genes Nutr. 5, 285–296 (2010).
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